A Bene Gesserit Reverend Mother who rises to lead the Sisterhood during its greatest crisis. Odrade is witty, unorthodox, and possessed of an Atreides bloodline that gives her dangerous impulses toward compassion and individual action - qualities the Bene Gesserit normally breed out.

A Bene Gesserit Bashar - supreme military commander - and one of the greatest tactical minds in human history. Teg is old, honourable, and devastatingly effective, a man whose reputation alone is a strategic asset. His loyalty to the Sisterhood is tested when the stakes escalate beyond anything his training prepared him for.

An unnamed senior Honored Matre encountered during the events of Heretics of Dune.

Bene Gesserit Mother Superior of the Gammu Keep where the Duncan Idaho ghola is raised. She secretly opposes Mother Superior Taraza's plans and conspires to have the ghola killed, believing the risk of an awakened Duncan is too great. She is killed during the Tleilaxu Face Dancer attack on the Keep.

A Tleilaxu Master who leads the conspiracy to control the sandworms through Sheeana. He represents the Tleilaxu's desperate bid for relevance as their axlotl tank technology becomes their only bargaining chip. He is killed on Rakis.

A heavyset, aggressive Bene Gesserit Mentat and Reverend Mother who serves as an advisor and deliberate counterweight to Mother Superior Odrade. She opposes Odrade's more merciful instincts and advocates ruthless action against both the Honored Matres and internal dissent. She becomes co-leader of the Sisterhood under Murbella.

Duncan Idaho is a loyal Atreides swordmaster, one of the finest fighters in the Imperium and deeply devoted to Duke Leto and his family. Trained by the Ginaz Swordmasters, Duncan combines exceptional combat ability with a charismatic personality and genuine warmth. He serves as weapons instructor to Paul Atreides and acts as the Duke's ambassador to the Fremen.

High Priest of Rakis and leader of the priesthood that controls access to the sandworms and Sheeana. He is manipulated by multiple factions and ultimately killed by Tleilaxu Face Dancers who replace him.

A Bene Gesserit Imprinter assigned to sexually bond the Duncan Idaho ghola to the Sisterhood's purposes. Skilled in seduction and combat, she escapes from Gammu with Burzmali and later plays a critical role in Chapterhouse by sharing the Lampadas memory horde with Rebecca before being killed by the Honored Matres.

Miles Teg's loyal aide and former military companion who has served alongside the Bashar for decades. He sacrifices himself to create a diversion, destroying Teg's former home on Lernaeus to cover Teg's escape from the Honored Matres.

A Tleilaxu Face Dancer - a shapeshifter capable of perfectly mimicking any human form. Scytale is an agent of the Bene Tleilax, involved in the conspiracy against Paul Muad'Dib, carrying both the threat of assassination and the temptation of resurrection.

A young Fremen girl from Rakis who can command the sandworms - the first person to demonstrate this ability since the age of Muad'Dib. Every faction in the galaxy wants to control Sheeana, but she proves far more capable of controlling her own destiny than anyone expects.

A Rakian priest involved in Tuek's inner circle who participates in the political manoeuvring around Sheeana and the worms.

An elderly Bene Gesserit archivist and Reverend Mother who serves on the Council alongside Bellonda and Odrade. She specialises in Bene Gesserit history and records. She dies at Junction during the Honored Matre assault.

Mother Superior of the Bene Gesserit during the events of Heretics of Dune. A brilliant political strategist who orchestrates the plan to use the Duncan Idaho ghola and Sheeana to control the sandworms of Rakis. She dies on Rakis during the Honored Matre attack, passing her memories to Darwi Odrade.

A Bene Gesserit operative and skilled fighter who assists Lucilla's escape from Gammu. He navigates the dangerous underground networks of the planet to get Lucilla to safety.

A Bene Gesserit sister stationed on Gammu who assists in the operation surrounding the Duncan Idaho ghola.

Failed Reverend Mother who served as Duncan Idaho's first teacher at the Gammu Keep. Developed genuine affection for the ghola child, which led to her reassignment.

A Bene Gesserit operative involved in the Gammu operations during the events of Heretics of Dune.